Plasmid

Part:BBa_K3505040:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-23)


pDGB1 alpha BACKBONE for Bacterial GoldenBraid Cloning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1274
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Judging form didn't let us to subbmit the whole plasmids for the Best Part Collection, because the whole plasmid had inside the restriction sites that made it not combatilbe for some Assembly. So we had to subbmit separatelly the backbone and the insert.